Ipatimup

RESEARCHDIAGNOSTICSTRANSLATIONOUTREACH

Joana Paredes

Name: Joana Paredes

E-mail: jparedes@i3s.up.pt

Joana Paredes

Member of

Coordinator of In vivo CAM Assays

Researcher of Cancer Metastasis

Short CV

Joana Paredes (JParedes) is the group leader of the Cancer Metastasis group at i3S and Affiliated Professor at the Faculty of Medicine of the University of Porto. Currently, she is also the President of the Portuguese Association for Cancer Research (ASPIC).

JParedes graduated in Biology at Coimbra University in 1999 and completed her PhD at the Faculty of Medicine of Porto University in 2004. For 2 years, she worked in the Cancer Research Laboratory of Gent University (Belgium), a reference lab in cell-cell adhesion and cancer.

Throughout JParedes’ scientific research career, she has been always committed to understand how cell-cell adhesion signaling regulates collective cell invasion, metabolic behavior and stem cell properties of cancer cells, supporting the use of adhesion molecules as prognostic and predictive biomarkers in cancer. As Principal Investigator, her research focus is to challenge the dogma that cell-cell adhesion needs to be lost for cancer cells to invade and metastasize. Using breast cancer as a model, she demonstrated that cell-cell adhesion mediated by P-cadherin provides invasive and stem-like properties to cancer cells. Recently, she showed that P-cadherin expression can be induced by hypoxia, which then activates the pentose phosphate pathway and decreases oxidative stress in matrix-detached breast cancer cells. These results led her to believe that cell-cell adhesion award plasticity and a survival advantage to cancer cells while in circulation, being determinant for an increased metastatic behavior.

JParedes has published 81 articles in international peer-reviewed journals and 4 book chapters. These manuscripts have gathered more than 3200 citations by peers, endowing her an h-index of 36. She already coordinated 14 and participated in 11 research projects and already acted as a reviewer of post-doc and PhD fellowships, as well as of scientific projects, for national and international agencies. As a mentor, JParedes has already supervised or co-supervised 8 PhD students and 17 MSc students.

Supervisor of

Ana Ribeiro, Assistant Researcher
Assistant Researcher

Bruno Freitas, PhD Student
PhD student

Catarina Lourenço, PhD Student
PhD student

Inês Conde, PhD Student
PhD student

Nuno Mendes, PhD Student
PhD student

Silvana Silva, Master Student (theoretical phase)
MSc student

PI (closed)

Metastização Cerebral: o impacto molecular e biomecânico das células tumorais no microambiente cerebral

Hoxome .: Função dos genes HOXB no Cancro de Mama

Tackling cancer stem cells: a challenge and an opportunity to advance in anti-cancer therapy (CANCELSTEM)

Length matters: Causes and consequences of centriole length deregulation in cancer

P-cadherin overexpression in invasive and metastatic basal-like breast cancer.

Combining siRNA and AAV therapy approaches to target human basal-like breast cancer: from vector development to anti-tumor efficacy evaluation

Adhesion, Differentiation and Invasion: different processes, common cadherin players

Dasatinib as an option to breat P-cadherin-overexpressing poor prognosis breast cancer

Wich is the role of both Cancer Stem Cells and Neural Stem Cells to the establishment of HER2+Breast Cancer Brain Metastases?

Basal-like Breast Cancer: are mammary stem cells new targets for cancer therapy?

Single-molecule analysis of cadherin-mediated cell-cell adhesion

First Author

1. Paredes J, Figueiredo J, Albergaria A, Oliveira P, Carvalho J, Ribeiro AS, Caldeira J, Costa AM, Simões-Correia J, Oliveira MJ, Pinheiro H, Pinho SS, Mateus R, Reis CA, Leite M, Fernandes MS, Schmitt F, Carneiro F, Figueiredo C, Oliveira C, Seruca R

Epithelial E- and P-cadherins: Role and clinical significance in cancer. Biochimica et Biophysica acta-Reviews on Cancer 1826: 297-311, 2012. [Review]

doi: 10.1016/j.bbcan.2012.05.002 PMID: 22613680.

160

9.0

2. Paredes J, Correia AL, Ribeiro AS, Milanezi F, Cameselle-Teijeiro J, Schmitt FC

Breast carcinomas that co-express E- and P-cadherin are associated with p120-catenin cytoplasmic localization and poor patient survival. Journal of clinical pathology 61: 856-62, 2008. [Article]

doi: 10.1136/jcp.2007.052704 PMID: 18381381.

2.3

3. Paredes J, Correia AL, Ribeiro AS, Albergaria A, Milanezi F, Schmitt FC

P-cadherin expression in breast cancer: a review. Breast cancer research : BCR 9: 214, 2007. [Review]

doi: 10.1186/bcr1774 PMID: 18001487.

4.4

4. Paredes J, Lopes N, Milanezi F, Schmitt FC

P-cadherin and cytokeratin 5: useful adjunct markers to distinguish basal-like ductal carcinomas in situ. Virchows Archiv : an international journal of pathology 450: 73-80, 2007. [Article]

doi: 10.1007/s00428-006-0334-y PMID: 17123107.

2.0

5. Paredes J, Correia AL, Ribeiro AS, Schmitt F

Expression of p120-catenin isoforms correlates with genomic and transcriptional phenotype of breast cancer cell lines. Cellular oncology : the official journal of the International Society for Cellular Oncology 29: 467-76, 2007. [Article]

PMID: 18032823.

4.2

6. Paredes J, Albergaria A, Oliveira JT, Jerónimo C, Milanezi F, Schmitt FC

P-cadherin overexpression is an indicator of clinical outcome in invasive breast carcinomas and is associated with CDH3 promoter hypomethylation. Clinical cancer research : an official journal of the American Association for Cancer Research 11: 5869-77, 2005. [Article]

doi: 10.1158/1078-0432.CCR-05-0059 PMID: 16115928.

192

5.7

7. Paredes J, Stove C, Stove V, Milanezi F, Van Marck V, Derycke L, Mareel M, Bracke M, Schmitt F

P-cadherin is up-regulated by the antiestrogen ICI 182,780 and promotes invasion of human breast cancer cells. Cancer research 64: 8309-17, 2004. [Article]

doi: 10.1158/0008-5472.CAN-04-0795 PMID: 15548699.

7.7

8. Paredes J, Milanezi F, Viegas L, Amendoeira I, Schmitt F

P-cadherin expression is associated with high-grade ductal carcinoma in situ of the breast. Virchows Archiv : an international journal of pathology 440: 16-21, 2002. [Article]

PMID: 11942571.

2.0

9. Paredes J, Milanezi F, Reis-Filho JS, Leitão D, Athanazio D, Schmitt F

Aberrant P-cadherin expression: is it associated with estrogen-independent growth in breast cancer? Pathology, research and practice 198: 795-801, 2002. [Article]

PMID: 12608656.

0.9

Senior Author

1. Conde I, Ribeiro AS, Paredes J

Breast Cancer Stem Cell Membrane Biomarkers: Therapy Targeting and Clinical Implications. Cells 11: ., 2022. [Article]

doi: 10.3390/cells11060934 PMID: 35326385.

7.7 (*)

2. Noronha C, Ribeiro AS, Taipa R, Castro DS, Reis J, Faria C, Paredes J

Cadherin Expression and EMT: A Focus on Gliomas. Biomedicines 9: ., 2021. [Review]

doi: 10.3390/biomedicines9101328 PMID: 34680444.

6.1 (*)

3. Pinto MT, Ribeiro AS, Conde I, Carvalho R, Paredes J

The Chick Chorioallantoic Membrane Model: A New In Vivo Tool to Evaluate Breast Cancer Stem Cell Activity. International journal of molecular sciences 22: ., 2020. [Article]

doi: 10.3390/ijms22010334 PMID: 33396951.

5.9

4. Sousa B, Pereira J, Marques R, Grilo LF, Pereira SP, Sardão VA, Schmitt F, Oliveira PJ, Paredes J

P-cadherin induces anoikis-resistance of matrix-detached breast cancer cells by promoting pentose phosphate pathway and decreasing oxidative stress. Biochimica et biophysica acta. Molecular basis of disease 1866: 165964, 2020. [Article]

doi: 10.1016/j.bbadis.2020.165964 PMID: 32920119.

4.4 (*)

5. Dionísio MR, Vieira AF, Carvalho R, Conde I, Oliveira M, Gomes M, Pinto MT, Pereira P, Pimentel J, Souza C, Marques MMC, Duval da Silva V, Barroso A, Preto D, Cameselle-Teijeiro JF, Schmitt F, Ribeiro AS, Paredes J

BR-BCSC Signature: The Cancer Stem Cell Profile Enriched in Brain Metastases that Predicts a Worse Prognosis in Lymph Node-Positive Breast Cancer. Cells 9: ., 2020. [Article]

doi: 10.3390/cells9112442 PMID: 33182375.

4.4 (*)

6. Sousa B, Pereira J, Paredes J

The Crosstalk Between Cell Adhesion and Cancer Metabolism. International journal of molecular sciences 20: ., 2019. [Review]

doi: 10.3390/ijms20081933 PMID: 31010154.

4.6

7. Sousa B, Ribeiro AS, Paredes J

Heterogeneity and Plasticity of Breast Cancer Stem Cells. Advances in experimental medicine and biology 1139: 83-103, 2019. [Article; Book Chapter]

doi: 10.1007/978-3-030-14366-4_5 PMID: 31134496.

2.5

8. Silva MR, Marques R, Sousa B, Sardao VA, Oliveira PJ, Paredes J

P-cadherin effect in mitochondrial dynamics and biogenesis of breast cancer cells European Journal of Clinical Investigation 49: 185-186, 2019. [Meeting Abstract]

3.5

9. Ribeiro AS, Nobre AR, Mendes N, Almeida J, Vieira AF, Sousa B, Carvalho FA, Monteiro J, Polónia A, Fonseca M, Sanches JM, Santos NC, Seruca R, Paredes J

SRC inhibition prevents P-cadherin mediated signaling and function in basal-like breast cancer cells. Cell communication and signaling : CCS 16: 75, 2018. [Article]

doi: 10.1186/s12964-018-0286-2 PMID: 30404626.

5.3 (*)

10. Polónia A, Pinto R, Cameselle-Teijeiro JF, Schmitt FC, Paredes J

Prognostic value of stromal tumour infiltrating lymphocytes and programmed cell death-ligand 1 expression in breast cancer. Journal of clinical pathology 70: 860-867, 2017. [Article]

doi: 10.1136/jclinpath-2016-203990 PMID: 28373294.

2.9

11. Vieira AF, Dionísio MR, Gomes M, Cameselle-Teijeiro JF, Lacerda M, Amendoeira I, Schmitt F, Paredes J

P-cadherin: a useful biomarker for axillary-based breast cancer decisions in the clinical practice. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 30: 698-709, 2017. [Article]

doi: 10.1038/modpathol.2016.232 PMID: 28084338.

6.7

12. Ribeiro AS, Carvalho FA, Figueiredo J, Carvalho R, Mestre T, Monteiro J, Guedes AF, Fonseca M, Sanches J, Seruca R, Santos NC, Paredes J

Atomic force microscopy and graph analysis to study the P-cadherin/SFK mechanotransduction signalling in breast cancer cells. Nanoscale 8: 19390-19401, 2016. [Article]

doi: 10.1039/c6nr04465d PMID: 27847941.

7.4

13. Vieira AF, Paredes J

P-cadherin and the journey to cancer metastasis. Molecular cancer 14: 178, 2015. [Review]

doi: 10.1186/s12943-015-0448-4 PMID: 26438065.

102

5.9

14. Ribeiro AS, Paredes J

P-Cadherin Linking Breast Cancer Stem Cells and Invasion: A Promising Marker to Identify an "Intermediate/Metastable" EMT State. Frontiers in oncology 4: 371, 2014. [Article]

doi: 10.3389/fonc.2014.00371 PMID: 25601904.

15. Vieira AF, Ribeiro AS, Dionísio MR, Sousa B, Nobre AR, Albergaria A, Santiago-Gómez A, Mendes N, Gerhard R, Schmitt F, Clarke RB, Paredes J

P-cadherin signals through the laminin receptor a6ß4 integrin to induce stem cell and invasive properties in basal-like breast cancer cells. Oncotarget 5: 679-92, 2014. [Article]

PMID: 24553076.

6.4

16. Sousa B, Ribeiro AS, Nobre AR, Lopes N, Martins D, Pinheiro C, Vieira AF, Albergaria A, Gerhard R, Schmitt F, Baltazar F, Paredes J

The basal epithelial marker P-cadherin associates with breast cancer cell populations harboring a glycolytic and acid-resistant phenotype. BMC cancer 14: 734, 2014. [Article]

doi: 10.1186/1471-2407-14-734 PMID: 25269858.

3.4

17. Ribeiro AS, Sousa B, Carreto L, Mendes N, Nobre AR, Ricardo S, Albergaria A, Cameselle-Teijeiro JF, Gerhard R, Söderberg O, Seruca R, Santos MA, Schmitt F, Paredes J

P-cadherin functional role is dependent on E-cadherin cellular context: a proof of concept using the breast cancer model. The Journal of pathology 229: 705-18, 2013. [Article]

doi: 10.1002/path.4143 PMID: 23180380.

7.3

18. Vieira AF, Ricardo S, Ablett MP, Dionísio MR, Mendes N, Albergaria A, Farnie G, Gerhard R, Cameselle-Teijeiro JF, Seruca R, Schmitt F, Clarke RB, Paredes J

P-cadherin is coexpressed with CD44 and CD49f and mediates stem cell properties in basal-like breast cancer. Stem cells (Dayton, Ohio) 30: 854-64, 2012. [Article]

doi: 10.1002/stem.1075 PMID: 22389315.

7.7

19. Schmitt F, Ricardo S, Vieira AF, Dionísio MR, Paredes J

Cancer stem cell markers in breast neoplasias: their relevance and distribution in distinct molecular subtypes. Virchows Archiv : an international journal of pathology 460: 545-53, 2012. [Review]

doi: 10.1007/s00428-012-1237-8 PMID: 22562130.

2.7

20. Ricardo S, Gerhard R, Cameselle-Teijeiro JF, Schmitt F, Paredes J

Claudin expression in breast cancer: High or low, what to expect? Histology and histopathology 27: 1283-95, 2012. [Article]

PMID: 22936447.

2.3

21. Ricardo S, Vieira AF, Gerhard R, Leitão D, Pinto R, Cameselle-Teijeiro JF, Milanezi F, Schmitt F, Paredes J

Breast cancer stem cell markers CD44, CD24 and ALDH1: expression distribution within intrinsic molecular subtype. Journal of clinical pathology 64: 937-46, 2011. [Article]

doi: 10.1136/jcp.2011.090456 PMID: 21680574.

413

2.3

22. Ribeiro AS, Albergaria A, Sousa B, Correia AL, Bracke M, Seruca R, Schmitt FC, Paredes J

Extracellular cleavage and shedding of P-cadherin: a mechanism underlying the invasive behaviour of breast cancer cells. Oncogene 29: 392-402, 2010. [Article]

doi: 10.1038/onc.2009.338 PMID: 19901964.

7.4

@ 2014 Ipatimup Institute of Molecular Pathology and Immunology of the University of Porto